Publication

研究業績

原著論文（Original papers）

  1. Shimada TL, Higaki T, Ebine K, Takano Y, Saijo Y, Ueda T (2026) Actin fragmentation induced by Colletotrichum higginsianum infection facilitates hyphal invasion in Arabidopsis thaliana leaves. Plant Cell Physiol in press (Published: 23 Mar 2026) https://doi.org/10.1093/pcp/pcag038.
  2. Hotta T, Pimm ML, Thomas EC, Yue Y, DeLear P, Blasius L, Cianfrocco MA, DeSantis ME, Horiuchi R, Higaki T, Sept D, Ohi R, Verhey KJ (2026) Accessibility of the unstructured alpha-tubulin C-terminal tail is controlled by microtubule lattice conformation. eLife. 14: RP109308. (Published: 9 Feb 2026) https://doi.org/10.7554/eLife.109308
  3. Ito K, Sonoda T, Hisanaga Y, Iwata KP, Goto C, Shirai K, Goto M, Yamamoto A, Mabuchi A, Higaki T, Song B, Negi J, Suzuki T, Fukaki H, Hanada K, Iba K, Kusumi K (2025) Arabidopsis LOHN1 peptide modulates lateral root spacing under the control of systemic nitrogen-satiety signaling. Curr Biol. in press. (Published: 24 Oct 2025) https://doi.org/10.1016/j.cub.2025.09.060
  4. Takahashi N, Ogita N, Koike T, Nishimura K, Inagaki S, Zhang Y, Higaki T, Umeda M (2025) The transcriptional repressors IAA5 and IAA29 participate in DNA damage-induced stem cell death in Arabidopsis roots. Plant Physiol in press. (Published: 4 Jul 2025) https://doi.org/10.1093/plphys/kiaf303
  5. Machfuudzoh A, Koshiba R, Higaki T, Ohba Y, Asahina M, Satoh S, Iwai H (2025) Jacalin-induced cell proliferation during tissue-repair process in partially incised flowering stems of Arabidopsis thaliana. Plant Cell Physiol in press. (Published: 1 Jul 2025) https://doi.org/10.1093/pcp/pcaf071
  6. Yoshida D, Kunita I, Toda M, H Ueda, Higaki T (2025) Four-dimensional phenotyping reveals MYOSIN XI-dependent establishment of branch morphology through upward- and stably-directed growth in Arabidopsis. Quant Plant Biol e15. (Published: 10 Jun 2025) https://doi.org/10.1017/qpb.2025.10007
  7. Hirase K, Higaki T (2025) Glycyrrhetinic acid triggers lipid peroxidation-related cell death in tobacco BY-2 cells. Biochem Biophys Res Commun 772: 152062. (Published: 21 May 2025) https://doi.org/10.1016/j.bbrc.2025.152062
  8. Soyama K, Miyakoshi A, Miyachi M, Itano M, Sugiyama H, Higaki T, Furukawa J, Iwai H (2025) Irregular swelling of tomato fruit chambers during the early stages of fruit formation is due to the local distribution of cell wall-loosening proteins under Ca-deficient conditions. J Plant Growth Regul in press. (Published: 20 May 2025) https://doi.org/10.1007/s00344-025-11745-0
  9. Kikuchi S, Kotaka T, Hanaki Y, Ueda M, Higaki T (2025) Distinct actin microfilament localization during early cell plate formation through deep learning-based image restoration. Plant Cell Rep 44: 115. (Published: 8 May 2025) https://doi.org/10.1007/s00299-025-03498-7
  10. Deng X, Higaki T, Lin HH, Lee YJ, Liu B (2025) The unconventional TPX2 family protein TPXL3 regulates α Aurora kinase function in spindle morphogenesis in Arabidopsis. Plant Cell in press. (Published: 26 Mar 2025) https://doi.org/10.1093/plcell/koaf065
  11. Ichita M, Yamamichi H, Higaki T (2025) Virtual staining from bright-field microscopy for label-free quantitative analysis of plant cell structures. Plant Mol Biol 115: 29. (Published: 31 Jan 2025) https://doi.org/10.1007/s11103-025-01558-w
  12. Takeda S, Nishikawa Y, Tachibana T, Higaki T, Sakamoto T, Kimura S (2025) Morphological and transcriptome analysis of the near-threatened orchid Habenaria radiata with petals shaped like a flying white bird. Plants 14: 393. (Published: 28 Jan 2025) https://doi.org/10.3390/plants14030393
  13. Horiuchi R, Kamimura A, Hanaki Y, Matsumoto H, Ueda M, Higaki T (2025) Deep learning-based cytoskeleton segmentation for accurate high-throughput measurement of cytoskeleton density. Protoplasma 262: 739-751. (Published: 18 Dec 2024) https://doi.org/10.1007/s00709-024-02019-9
  14. Hitora Y, Hokaguchi M, Sadahiro Y, Higaki T, Tsukamoto S (2024) Machine learning accelerates screening of osteoclast differentiation inhibitors from natural products. J Nat Prod. 87: 2393–2397. (Published: 4 Oct 2024) https://doi.org/10.1021/acs.jnatprod.4c00640
  15. Notaguchi M, Ichita M, Kawasoe T, Monda K, Kurotani K, Higaki T, Iba K, Hashimoto-Sugimoto M (2024) The PATROL1 function in roots contributes to the increase in shoot biomass. Planta 260: 105. (Published: 26 Sep 2024) https://doi.org/10.1007/s00425-024-04526-8
  16. Tanaka S, Matsushita Y, Hanaki Y, Higaki T, Kamamoto N, Matsushita K, Higashiyama T, Fujimoto K, Ueda M (2024) HD-ZIP IV genes are essential for embryo initial cell polarization and the radial axis formation in Arabidopsis. Curr Biol 34: 4639–4649. (Published: 19 Sep 2024) https://doi.org/10.1016/j.cub.2024.08.038
  17. Machfuudzoh A, Pitaksaringkarn W, Koshiba R, Higaki T, Rakwal R, Ohba Y, Asahina M, Satoh S, Iwai H (2024) At2-MMP is required for attenuation of cell proliferation during wound healing in incised Arabidopsis inflorescence stems. Plant Cell Physiol 65: 1821–1832. (Published: 13 Sep 2024) https://10.1093/pcp/pcae103
  18. Ezaki K, Koga H, Takeda-Kamiya N, Toyooka K, Higaki T, Sakamoto S, Tsukaya H (2024) Precocious cell differentiation occurs in proliferating cells in leaf primordia in Arabidopsis angustifolia3 mutant. Front Plant Sci 15: 1322223. (Published: 16 Apr 2024) https://doi.org/10.3389/fpls.2024.1322223
  19. Hitora Y, El-Desoky AH, Sadahiro Y, Sejiyama A, Kinoshita A, Ise Y, Angkouw ED, Mangindaan REP, Higaki T, Tsukamoto S (2024) Neopetromin, a cyclic tripeptide with a C-N cross-link, from the marine sponge Neopetrosia sp., that causes vacuole fragmentation in tobacco BY-2 cells. J Nat Prod. 87: 1197–1202. (Published: 19 Mar 2024) https://doi.org/10.1021/acs.jnatprod.4c00158
  20. Hiromoto Y, Minamino N, Kikuchi S, Kimata Y, Matsumoto H, Nakagawa S, Ueda M, Higaki T (2023) Comprehensive and quantitative analysis of intracellular structure polarization at the apical-basal axis in elongating Arabidopsis zygotes. Sci Rep 13: 22879. (Published: 18 Dec 2023) https://doi.org/10.1038/s41598-023-50020-8
  21. Kikukawa K, Takigawa-Imamura H, Soga K, Kotake T, Higaki T (2023) Smooth elongation of pavement cells induced by RIC1 overexpression leads to marginal protrusions of the cotyledon in Arabidopsis thaliana. Plant Cell Physiol 64: 1356–1371. (Published: 18 Sep) https://doi.org/10.1093/pcp/pcad094
  22. Hirano T, Ebine K, Ueda T, Higaki T, Watanabe-Nakayama T, Konno H, Takigawa-Imamura H, Sato MH (2023) The SYP123-VAMP727 SNARE complex delivers secondary cell wall components for root hair shank hardening in Arabidopsis. Plant Cell 35: 4347–4365. (Published: 15 Sep 2023) https://doi.org/10.1093/plcell/koad240
  23. Yue Y, Hotta T, Higaki T, Verhey KJ, Ohi R (2023) Microtubule detyrosination by VASH1/SVBP is regulated by the conformational state of tubulin in the lattice. Curr Biol 33: 4111–412. (Published: 15 Sep 2023) https://doi.org/10.1016/j.cub.2023.07.062
  24. Matsumoto T, Higaki T, Takatsuka H, Kutsuna N, Ogata Y, Hasezawa S, Umeda M, Inada N (2023) Arabidopsis thaliana subclass I ACTIN DEPOLYMERIZING FACTORs regulate nuclear organization and gene expression. Plant Cell Physiol 64: 1231–1242. (Published: 18 Aug 2023) https://doi.org/10.1093/pcp/pcad092
  25. Nishimura T, Mori S, Shikata H, Nakamura M, Hashiguchi Y, Abe Y, Hagihara T, Yoshikawa HY, Toyota M, Higaki T, Morita MT (2023) Cell polarity linked to gravity sensing is generated by protein translocation from statoliths to the plasma membrane. Science. 381: 1006–1010. (Published: 11 Aug 2023) https://doi.org/10.1126/science.adh9978
  26. Wint H, Li J, Abe T, Yamada H, Higaki T, Nasu Y, Watanabe M, Takei K, Takeda T (2023) Pacsin 2-dependent N-cadherin internalization regulates the migration behaviour of malignant cancer cells. J Cell Sci. 136: jcs260827. (Published: 3 May 2023) https://doi.org/10.1242/jcs.260827
  27. Hirano T, Okamoto A, Oda Y, Sakamoto T, Takeda S, Matsuura T, Ikeda Y, Higaki T, Kimura S, Sato MH (2023) Ab-GALFA, A bioassay for insect gall formation using the model plant Arabidopsis thaliana. Sci Rep. 13:2554. (Published: 13 February 2023) https://doi.org/10.1038/s41598-023-29302-8
  28. Morotomi-Yano K, Hiromoto Y, Higaki T, Yano KI (2022) Disease-associated H58Y mutation affects the nuclear dynamics of human DNA topoisomerase IIβ. Sci Rep 12: 20627. (Published: 30 November 2022)  https://doi.org/10.1038/s41598-022-24883-2
  29. Yoshida D, Akita K, Higaki T (2022) Machine learning and feature analysis of the cortical microtubule organization of Arabidopsis cotyledon pavement cells. Protoplasma 260: 987-998. (Published: 11 October 2022) https://doi.org/10.1007/s00709-022-01813-7
  30. Hotta T, McAlear TS, Yue Y, Higaki T, Haynes SE, Nesvizhskii AI, Sept D, Verhey KJ, Bechstedt S, Ohi R (2022) EML2-S constitutes a new class of proteins that recognizes and regulates the dynamics of tyrosinated microtubules. Curr Biol 32: 3898-3910. (Published: 12 August 2022) https://doi.org/10.1016/j.cub.2022.07.027
  31. Takihara Y, Higaki T, Yokomizo T, Umemoto T, Ariyoshi K, Hashimoto M, Sezaki M, Takizawa H, Inoue T, Suda T, Mizuno H (2022) Bone marrow imaging reveals the migration dynamics of neonatal hematopoietic stem cells. Commun Biol 5: 776. (Published: 02 August 2022) https://doi.org/10.1038/s42003-022-03733-x
  32. Sakamoto T, Sakamoto Y, Grob S, Slane D, Yamashita T, Ito N, Oko Y, Sugiyama T, Higaki T, Hasezawa S, Tanaka M, Matsui A, Seki M, Suzuki T, Grossniklaus U, Matsunaga S (2022) Two-step regulation of centromere distribution by condensin II and the nuclear envelope proteins. Nat Plants 8: 940–953. (Published: 01 August 2022) https://doi.org/10.1038/s41477-022-01200-3
  33. Hotta T, Lee YR, Higaki T, Hashimoto T, Liu B (2022) Two Kinesin-14A motors oligomerize to drive poleward microtubule convergence for acentrosomal spindle morphogenesis in Arabidopsis thaliana. Front Cell Dev Biol. 10:949345. (Published: 22 July 2022) https://doi.org/10.3389/fcell.2022.949345 
  34. Sakai Y, Higaki T, Ishizaki K, Nishihama R, Kohchi T, Hasezawa S (2022) Migration of prospindle before the first asymmetric division in germinating spore of Marchantia polymorpha. Plant Biotech 39: 5-12. (Published: 25 Mar 2022) https://doi.org/10.5511/plantbiotechnology.21.1217b
  35. Okubo-Kurihara E, Ali A, Hiramoto M, Kurihara Y, Abouleila Y, Abdelazem EM, Kawai T, Makita Y, Kawashima M, Esaki T, Shimada H, Mori T, Hirai MY, Higaki T, Hasezawa S, Shimizu Y, Masujima T, Matsui M (2022) Tracking metabolites at single-cell resolution reveals metabolic dynamics during plant mitosis. Plant Physiol 189: 459-464. (Published: 18 Mar 2022) https://doi.org/10.1093/plphys/kiac093
  36. Suzuki R, Yamada M, Higaki T, Aida M, Kubo M, Tsai AY, Sawa S (2021) PUCHI regulates giant cell morphology during root-knot nematode infection in Arabidopsis thaliana. Front Plant Sci 12: 755610. (Published: 06 Oct 2021) https://doi.org/10.3389/fpls.2021.755610
  37. Kikukawa K, Yoshimura K, Watanabe A, Higaki T (2021) Metal-nano-ink coating for monitoring and quantification of cotyledon epidermal cell morphogenesis. Front Plant Sci 12: 745980. (Published: 21 Sep 2021) https://doi.org/10.3389/fpls.2021.745980
  38. Kamon E, Noda C, Higaki T, Demura T, Ohtani M (2021) Calcium signaling contributes to xylem vessel cell differentiation via post-transcriptional regulation of VND7 downstream events. Plant Biotech 38: 331-337 (Published: 18 Sep 2021) https://doi.org/10.5511/plantbiotechnology.21.0519a
  39. Fujihara R, Uchida N, Tameshige T, Kawamoto N, Hotokezaka Y, Higaki T, Simon R, Torii KU, Tasaka M, Aida M (2021) The boundary-expressed EPIDERMAL PATTERNING FACTOR-LIKE2 gene encoding a signaling peptide promotes cotyledon growth during Arabidopsis thaliana embryogenesis. Plant Biotech 38: 317-322. (Published: 18 Sep 2021) https://doi.org/10.5511/plantbiotechnology.21.0508a
  40. Sato F, Iba K, Higaki T (2021) Involvement of the membrane trafficking factor PATROL1 in the salinity stress tolerance of Arabidopsis thaliana. Cytologia 86: 119-126. (Published: 25 June 2021) https://doi.org/10.1508/cytologia.86.119
  41. Higaki T, Sato F, Iba K (2021) Environmental responses of the membrane trafficking factor PATROL1 in the Arabidopsis stomatal complex. Cytologia 86: 101-102. (Published: 25 June 2021) https://doi.org/10.1508/cytologia.86.102
  42. Kimura T, Haga K, Nomura Y, Higaki T, Nakagami H, Sakai T (2021) Phosphorylation of NONPHOTOTROPIC HYPOCOTYL3 affects photosensory adaptation during the phototropic response. Plant Physiol 187: 981–995. (Published: 17 June 2021) https://doi.org/10.1093/plphys/kiab281
  43. Matsumoto H, Kimata Y, Higaki T, Higashiyama T, Ueda M (2021) Dynamic rearrangement and directional migration of tubular vacuoles are required for the asymmetric division of the Arabidopsis zygote. Plant Cell Physiol 62: 1280–1289. (Published: 02 Jun 2021) https://doi.org/10.1093/pcp/pcab075
  44. Kunita I, Morita MT, Toda M, Higaki T (2021) A three-dimensional scanning system for digital archiving and quantitative evaluation of Arabidopsis plant architectures. Plant Cell Physiol 62: 1975-1982. (Published: 22 May 2021) https://doi.org/10.1093/pcp/pcab068
  45. Ishida T, Yoshimura H, Takekawa M, Higaki T, Ideue T, Hatano M, Igarashi M, Tani T, Sawa S, Ishikawa H (2021) Discovery, characterization and functional improvement of kumamonamide as a novel plant growth inhibitor that disturbs plant microtubules. Sci Rep 23: 6077. (Published: 23 Mar 2021) http://doi.org/10.1038/s41598-021-85501-1
  46. Maeda K, Higaki T (2021) Disruption of actin filaments delays accumulation of cell plate membranes after chromosome separation. Plant Sig Behav 16: 1873586. (Published: 11 Jan 2021) https://doi.org/10.1080/15592324.2021.1873586
  47. Higaki T, Akita K, Katoh K (2020) Coefficient of variation as an image-intensity metric for cytoskeleton bundling. Sci Rep 10: 22187. (Published: 21 Dec 2020) https://doi.org/10.1038/s41598-020-79136-x
  48. Lu YJ, Li P, Shimono M, Corrion A, Higaki T, He SY, Day B (2020) Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity. Nat Commun 11:6234. (Published: 04 Dec 2020) https://doi.org/10.1038/s41467-020-20007-4
  49. Kimata Y, Higaki T, Kurihara D, Ando N, Matsumoto H, Higashiyama T, Ueda M (2020) Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes. Quant Plant Biol 1: e3. (Published: 30 Nov 2020) https://doi.org/10.1017/qpb.2020.4
  50. Higaki T, Mizuno H (2020) Four-dimensional imaging with virtual reality to quantitatively explore jigsaw puzzle-like morphogenesis of Arabidopsis cotyledon pavement cells. Plant Biotech 37: 429-435. (Published: 05 Sep 2020) http://doi.org/10.5511/plantbiotechnology.20.0605a
  51. Yoshimi Y, Hara K, Yoshimura M, Tanaka N, Higaki T, Tsumuraya Y, Kotake T (2020) Expresion of a fungal exo-β-1,3-galactanase in Arabidopsis reveals a role of type II arabinogalactans in the regulation of cell shape. J Exp Bot 71: 5414-5424. (Published: 29 May 2020) https://doi.org/10.1093/jxb/eraa236
  52. Higaki T, Akita K, Hasezawa S (2020) Elevated CO2 promotes satellite stomata production in young cotyledons of Arabidopsis thaliana. Genes Cells 25: 475-482. (Published: 15 Apr 2020) https://doi.org/10.1111/gtc.12773
  53. Kwon SH, Wi T, Park YI, Kim MW, Lee G, Higaki T, Choi JH, Lee R (2020) Non-invasive early detection of calpain 2-enriched non-small cell lung cancer using a human serum albumin-bounded calpain 2 nanosensor. Bioconjug Chem 31: 803-812. (Published: 18 Feb 2020) https://doi.org/10.1021/acs.bioconjchem.9b00870
  54. Maeda K, Sasabe M, Hanamata S, Machida Y, Hasezawa S, Higaki T (2020) Actin filament disruption alters phragmoplast microtubule dynamics during the initial phase of plant cytokinesis. Plant Cell Physiol 61: 445-456. (Published: 07 Feb 2020) https://doi.org/10.1093/pcp/pcaa003
  55. Hirai R, Higaki T, Takenaka Y, Sakamoto Y, Hasegawa J, Matsunaga S, Demura T, Ohtani M (2020) The progression of xylem vessel cell differentiation is dependent on the activity level of VND7 in Arabidopsis thaliana. Plants 9: 39. (Published: 25 Dec 2019) https://doi.org/10.3390/plants9010039
  56. Wong JH, Kato T, Belteton S, Shimizu R, Kinoshita N, Higaki T, Sakumura Y, Szymanski DB, Hashimoto T (2019) Basic proline-rich protein-mediated microtubules are essential for lobe growth and flattened cell geometry. Plant Physiol 181: 1535-1551. (Published: 25 Nov 2019) https://doi.org/10.1104/pp.19.00811
  57. Akita K, Higaki T (2019) An induction system for clustered stomata by sugar solution immersion treatment in Arabidopsis thaliana seedlings. J Vis Exp 144: e58951. (Published: 15 Feb 2019) http://doi.org/10.3791/58951
  58. Nagashima A, Higaki T, Koeduka T, Ishigami K, Hosokawa S, Watanabe H, Matsui K, Hasezawa S, Touhara K (2019) Transcriptional regulators involved in responses to volatile organic compounds in plants. J Biol Chem 294: 2256-2266. (Published: 15 Feb 2019) http://doi.org/10.1074/jbc.RA118.005843
  59. Kimata Y, Kato T, Higaki T, Kurihara D, Yamada T, Segami S, Morita MT, Maeshima M, Hasezawa S, Higashiyama T, Tasaka M, Ueda M (2019) Polar vacuolar distribution is essential for accurate asymmetric division of Arabidopsis zygotes. Proc Natl Acad Sci U S A 116: 2338-2343. (Published: 05 Feb 2019) https://doi.org/10.1073/pnas.1814160116
  60. Miyoshi S, Kimura S, Ootsuki R, Higaki T, Nakamasu A (2019) Developmental analyses of divarications in leaves of an aquatic fern Microsorum pteropus and its varieties. PLOS One 14: e0210141. (Published: 25 Jan 2019) http://doi.org/10.1371/journal.pone.0210141
  61. Tanaka M, Fujii Y, Hirano K, Higaki T, Nagasaki A, Ishikawa R, Okajima T, Katoh K (2019) Fascin in lamellipodia contributes to cell elasticity by controlling the orientation of filamentous actin. Genes Cells 24: 202-213. (Published: 21 Jan 2019) https://doi.org/10.1111/gtc.12671
  62. Yi-Lun Tsai A, Higaki T, Nguyen CN, Perfus-Barbeoch L, Favery B, Sawa S (2018) Regulation of root-knot nematode behavior by seed coat mucilage-derived attractants. Mol Plant 12: 99-112. (Published: 28 Nov 2018) https://doi.org/10.1016/j.molp.2018.11.008
  63. Takatsuka H, Higaki T, Umeda M (2018) Actin reorganization triggers rapid cell elongation in Arabidopsis roots. Plant Physiol 178: 1130-1141. (Published: 13 Nov 2018) http://doi.org/10.1104/pp.18.00557
  64. Hirano T, Konno H, Takeda S, Dolan L, Kato M, Aoyama T, Higaki T, Takigawa-Imamura H, Sato MH (2018) PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis. Nat Plants 4: 888-897. (Published: 02 Nov 2018) http://doi.org/10.1038/s41477-018-0277-8.
  65. Hirakawa Y, Hasezawa S, Higaki T (2018) Reactive oxygen species production and stimulated endocytosis in tobacco BY-2 cells treated with Erwinia carotovora culture filtrate. Cytologia 83: 289-293. (Published: 25 Sep 2018) https://doi.org/10.1508/cytologia.83.289
  66. Akita K, Hasezawa S, Higaki T (2018) Cortical microtubules and fusicoccin response in clustered stomatal guard cells induced by sucrose solution immersion. Plant Sig Behav 13: 1454815. (Published: 01 May 2018) https://doi.org/10.1080/15592324.2018.1454815
  67. Dou L, He K, Higaki T, Wang X, Mao T (2018) Ethylene signaling modulates cortical microtubule reassembly in response to salt stress. Plant Physiol 176: 2071-2081. (Published: 05 Feb 2018) https://doi.org/10.1104/pp.17.01124
  68. Kuki H, Higaki T, Yokoyama R, Kuroha T, Shinohara N, Hasezawa S, Nishitani K (2017) Quantitative confocal imaging method for analyzing cellulose dynamics during cell wall regeneration in Arabidopsis mesophyll protoplasts. Plant Direct e00021. (Published: 27 Dec 2017) https://doi.org/10.1002/pld3.21
  69. Takahashi S, Monda K, Higaki T, Hashimoto-Sugimoto M, Negi J, Hasezawa S, Iba K (2017) Differential effects of phosphatidylinositol 4-Kinase (PI4K) and 3-Kinase (PI3K) inhibitors on stomatal responses to environmental signals. Front Plant Sci 8: 677. (Published: 01 May 2017) http://doi.org/10.3389/fpls.2017.00677
  70. Fukunaga H, Arita T, Higaki T, Sawa S (2017) A new form of Gastrodia pubilabiata (Orchidaceae). Acta Phytotax et Geobot 68: 45-52. (Published: 28 Feb 2017) https://doi.org/10.18942/apg.201613
  71. Higaki T, Takigawa-Imamura H, Akita K, Kutsuna N, Kobayashi R, Hasezawa S, Miura T (2017) Exogenous cellulase switches cell interdigitation to cell elongation in a RIC1-dependent manner in Arabidopsis thaliana cotyledon pavement cells. Plant Cell Physiol 58: 106-119. (Published: 23 Dec 2016) http://doi.org/10.1093/pcp/pcw183
  72. Takahashi M, Umetsu K, Oono Y, Higaki T, Blancaflor E, Rahman A (2017) SMALL ACIDIC PROTEIN 1 (SMAP1) and SCFTIR1 ubiquitin proteasome pathway act in concert to induce 2,4-dichlorophenoxyacetic acid-mediated alteration of actin in Arabidopsis roots. Plant J 89: 940-956. (Published: 25 Nov 2016) http://doi.org/10.1111/tpj.13433
  73. Kimata Y, Higaki T, Kawashima T, Kurihara D, Sato Y, Yamada T, Hasezawa S, Berger F, Higashiyama T, Ueda M (2016) Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote. Proc Natl Acad Sci U S A 113: 14157-14162. (Published: 22 Nov 2016) http://doi.org/10.1073/pnas.1613979113
  74. Shimono M, Higaki T, Kaku H, Shibuya N, Hasezawa S, Day B (2016) Quantitative evaluation of stomatal cytoskeletal patterns during the activation of immune signaling in Arabidopsis thaliana. PLOS One 11: e0159291. (Published: 14 Jul 2016) http://doi.org/10.1371/journal.pone.0159291
  75. Higaki T, Kutsuna N, Akita K, Takigawa-Imamura H, Yoshimura K, Miura T (2016) A theoretical model of jigsaw-puzzle pattern formation by plant leaf epidermal cells. PLOS Comput Biol 12: e1004833. (Published: 07 Apr 2016) http://doi.org/10.1371/journal.pcbi.1004833
  76. Hashimoto-Sugimoto M, Negi J, Monda K, Higaki T, Isogai Y, Nakano T, Hasezawa S, Iba K (2016) Dominant and recessive mutations in the Raf-like kinase HT1 gene completely disrupt the stomatal responses to CO2. J Exp Bot 67: 3251-3261. (Published: 31 Mar 2016) http://doi.org/10.1093/jxb/erw134
  77. Akita K, Kobayashi M, Sato M, Kutsuna N, Ueda T, Toyooka K, Nagata N, Hasezawa S, Higaki T (2017) Cell wall accumulation of fluorescent proteins derived from a trans-Golgi cisternal membrane marker and paramural bodies in interdigitated Arabidopsis leaf epidermal cells. Protoplasma 254: 367-377. (Published: 09 Mar 2016) http://doi.org/10.1007/s00709-016-0955-1
  78. Inada N, Higaki T, Hasezawa S (2016) Nuclear function of subclass I actin depolymerizing factor contributes to susceptibility in Arabidopsis to an adapted powdery mildew fungus. Plant Physiol 170: 1420-1434. (Published: 08 Jan 2016) http://doi.org/10.1104/pp.15.01265
  79. Inada N, Higaki T, Hasezawa S (2016) Quantitative analyses on dynamic changes in the organization of host Arabidopsis thaliana actin microfilaments surrounding the infection organ of the powdery mildew fungus Golovinomyces orontii. J Plant Res 129: 103-110. (Published: 08 Dec 2015) http://doi.org/10.1007/s10265-015-0769-9
  80. Akita K, Higaki T, Kutsuna N, Hasezawa S (2015) Quantitative analysis of microtubule orientation in interdigitated leaf pavement cells. Plant Signal Behav 10: e1024396. (Published: 03 Jun 2015) http://doi.org/10.1080/15592324.2015.1024396
  81. Hirakawa Y, Nomura T, Hasezawa S, Higaki T (2015) Simplification of vacuole structure during plant cell death triggered by culture filtrates of Erwinia carotovora. J Integr Plant Biol 57: 127-135. (Published: 31 Oct 2014) http://doi.org/10.1111/jipb.12304
  82. Higaki T, Kutsuna N, Akita K, Sato M, Sawaki F, Kobayashi M, Nagata N, Toyooka K, Hasezawa S (2015) Semi-automatic organelle detection on transmission electron microscopic images. Sci Rep 5: 7794. (Published: 15 Jan 2015) http://doi.org/10.1038/srep07794
  83. Higaki T, Kato A, Myouga F, Kutsuna N, Hasezawa S, Nagata N (2014) Automatic classification of chloroplast ultrastructure mutants with transmission electron microscopy. Bioimages 22: 1-7. (J-stage Released: 30 Jun  2015)  https://doi.org/10.11169/bioimages.22.1
  84. Hasegawa J, Higaki T, Hamamura Y, Kurihara D, Kutsuna N, Higashiyama T, Hasezawa S, Matsunaga S (2014) Increase in invaginated vacuolar membrane structure caused by plant cell expansion by genotoxic stress induced by DNA double-strand breaks. Cytologia 79: 467-474. (Published: 25 Dec 2014) https://doi.org/10.1508/cytologia.79.467
  85. Toyooka K, Sato M, Kutsuna N, Higaki T, Sawaki F, Wakazaki M, Goto Y, Hasezawa S, Nagata N, Matsuoka K (2014) Wide-range high-resolution transmission electron microscopy reveals morphological and distributional changes of endomembrane compartments during log-to-stationary transition of growth phase in tobacco BY-2 cells. Plant Cell Physiol 55: 1544-1555. (Published: 13 Jun 2014) http://doi.org/10.1093/pcp/pcu084
  86. Higaki T, Higaki S, Hirota M, Hasezawa S (2014) Radiocesium distribution in bamboo shoots after the Fukushima nuclear accident. PLOS One 9: e97659. (Published: 15 May 2014) http://doi.org/10.1371/journal.pone.0097659
  87. Hirota M, Higaki S, Higaki T, Hasezawa S (2013) Investigation of contamination in spinach collected immediately following the Fukushima Daiichi nuclear disaster. Rad Safe Manager 12: 43-47. (J-stage Released: 19 Dec 2013) https://doi.org/10.12950/rsm.12.43
  88. Higaki T, Hashimoto-Sugimoto M, Akita K, Iba K, Hasezawa S (2014) Dynamics and environmental responses of PATROL1 in Arabidopsis subsidiary cells. Plant Cell Physiol 55: 773-780. (Published: 07 Nov 2013) http://doi.org/10.1093/pcp/pct151
  89. Akita K, Hasezawa S, Higaki T (2013) Breaking of the plant stomatal one-cell-spacing rule by sugar solution immersion. PLOS One 8: e72456. (Published: 11 Sep 2013) http://doi.org/10.1371/journal.pone.0072456
  90. Hashimoto-Sugimoto M, Higaki T, Yaeno T, Nagami A, Irie M, Fujimi M, Miyamoto M, Akita K, Negi J, Shirasu K, Hasezawa S, Iba K (2013) A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that is essential for stomatal responses. Nat Commun 4: 2215. (Published: 30 Jul 2013) http://doi.org/10.1038/ncomms3215
  91. Kojo KH, Higaki T, Kutsuna N, Yoshida Y, Yasuhara H, Hasezawa S (2013) Roles of cortical actin microfilament patterning in division plane orientation in plants. Plant Cell Physiol 54: 1491-1503. (Published: 27 Jul 2013) http://doi.org/10.1093/pcp/pct093
  92. Higaki T, Kutsuna N, Hasezawa S (2013) LIPS database with LIPService: a microscopic image database of intracellular structures in Arabidopsis guard cells. BMC Plant Biol 13: 81. (Published: 16 May 2013) http://doi.org/10.1186/1471-2229-13-81
  93. Kutsuna N*, Higaki T*, Matsunaga S*, Otsuki T, Yamaguchi M, Fujii H, Hasezawa S (2012) Active learning framework with iterative clustering for bioimage classification. Nat Commun 3: 1032. (*equal contribution) (Published: 28 Aug 2012) http://doi.org/10.1038/ncomms2030
  94. Era A, Kutsuna N, Higaki T, Hasezawa S, Nakano A, Ueda T (2013) Microtubule stability affects the unique motility of F-actin in Marchantia polymorpha. J Plant Res 126: 113-119. (Published: 08 Jun 2012) http://doi.org/10.1007/s10265-012-0496-4
  95. Higaki T, Kutsuna N, Hosokawa Y, Akita K, Ebine K, Ueda T, Kondo N, Hasezawa S (2012) Statistical organelle dissection of Arabidopsis guard cells using image database LIPS. Sci Rep 2: 405. (Published: 11 May 2012) http://doi.org/10.1038/srep00405
  96. Higaki T, Higaki S, Hirota M, Akita K, Hasezawa S (2012) Radionuclide analysis on bamboos following the Fukushima nuclear accident. PLOS One 7: e34766. (Published: 04 Apr 2012) http://doi.org/10.1371/journal.pone.0034766
  97. Okubo-Kurihara E, Higaki T, Kurihara Y, Kutsuna N, Yamaguchi J, Hasezawa S (2010) Sucrose transporter NtSUT4 from tobacco BY-2 involved in plant cell shape during miniprotoplast culture. J Plant Res 124: 395-403. (Published: 13 Oct 2010) http://doi.org/10.1007/s10265-010-0377-7
  98. Yoneda A, Ito T, Higaki T, Kutsuna N, Saito T, Ishimizu T, Osada H, Hasezawa S, Matsui M, Demura T (2010) Cobtorin target analysis reveals that pectin functions in the deposition of cellulose microfibrils in parallel with cortical microtubules. Plant J 64: 657-667. (Published: 15 September 2010) http://doi.org/10.1111/j.1365-313X.2010.04356.x
  99. Higaki T, Kutsuna N, Sano T, Kondo N, Hasezawa S (2010) Quantification and cluster analysis of actin cytoskeletal structures in plant cells: role of actin bundling in stomatal movement during diurnal cycles in Arabidopsis guard cells. Plant J 61: 156-165. (Published: 18 Dec 2009) http://doi.org/10.1111/j.1365-313x.2009.04032.x
  100. Nagano AJ, Maekawa A, Nakano RT, Miyahara M, Higaki T, Kutsuna N, Hasezawa S, Hara-Nishimura I (2009) Quantitative analysis of ER body morphology in an Arabidopsis mutant. Plant Cell Physiol 50: 2015-2022. (Published: 24 Sep 2009) http://doi.org/10.1093/pcp/pcp157
  101. Okubo-Kurihara E, Sano T, Higaki T, Kutsuna N, Hasezawa S (2009) Acceleration of vacuolar regeneration and cell growth by overexpression of an aquaporin NtTIP1;1 in tobacco BY-2 cells. Plant Cell Physiol 50: 151-160. (Published: 27 Nov 2008) http://doi.org/10.1093/pcp/pcn181
  102. Higaki T, Kadota Y, Goh T, Hayashi T, Kutsuna N, Sano T, Hasezawa S, Kuchitsu K (2008) Vacuolar and cytoskeletal dynamics during elicitor-induced programmed cell death in tobacco BY-2 cells. Plant Sig Behav 3: 700-703. (Published: 01 Sep 2008) http://doi.org/10.4161/psb.3.9.6431
  103. Higaki T, Kutsuna N, Sano T, Hasezawa S (2008) Quantitative analysis of changes in actin microfilament contribution to cell plate development in plant cytokinesis. BMC Plant Biol 8: 80. (Published: 17 Jul 2008) http://doi.org/10.1186/1471-2229-8-80
  104. Higaki T, Goh T, Hayashi T, Kutsuna N, Kadota Y, Hasezawa S, Sano T, Kuchitsu K (2007) Elicitor-induced cytoskeletal rearrangement relates to vacuolar dynamics and execution of cell death: in vivo imaging of hypersensitive cell death in tobacco BY-2 cells. Plant Cell Physiol 48: 1414-1425. (Published: 01 Oct 2007) http://doi.org/10.1093/pcp/pcm109
  105. Yoneda A, Higaki T, Kutsuna N, Kondo Y, Osada H, Hasezawa S, Matsui M (2007) Chemical genetic screening identifies a novel inhibitor of parallel alignment of cortical microtubules and cellulose microfibrils. Plant Cell Physiol 48: 1393-1403. (Published: 01 Oct 2007) http://doi.org/10.1093/pcp/pcm120
  106. Gambe AG, Maniwa R, Matsunaga S, Kutsuna N, Higaki T, Higashi T, Hasezawa S, Uchiyama S, Fukui K (2007) Development of a multistage classifier for a monitoring system of cell activity based on imaging of chromosomal dynamics. Cytometry A 71: 286-296. (Published: 12 Mar 2007) http://doi.org/10.1002/cyto.a.20378
  107. Higaki T, Kutsuna N, Okubo E, Sano T, Hasezawa S (2006) Actin microfilaments regulate vacuolar structures and dynamics: dual observation of actin microfilaments and vacuolar membrane in living tobacco BY-2 cells. Plant Cell Physiol 47: 839-852. (Published: 01 Jul 2006) http://doi.org/10.1093/pcp/pcj056
  108. Sano T, Higaki T, Handa K, Kadota Y, Kuchitsu K, Hasezawa S, Hoffmann A, Endter J, Zimmermann U, Hedrich R, Roitsch T (2006) Calcium ions are involved in the delay of plant cell cycle progression by abiotic stresses. FEBS Lett 580: 597-602. (Published: 04 Jan 2006) http://doi.org/10.1016/j.febslet.2005.12.074
  109. Sano T*, Higaki T*, Oda Y, Hayashi T, Hasezawa S (2005) Appearance of actin microfilament ‘twin peaks’ in mitosis and their function in cell plate formation, as visualized in tobacco BY-2 cells expressing GFP-fimbrin. Plant J 44: 595-605. (*equal contribution) (Published: 14 Oct 2005) http://doi.org/10.1111/j.1365-313X.2005.02558.x